| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
Essential Fatty Acid Assimilation and Synthesis in Larvae of the Bivalve Crassostrea gigas
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| Autores: |
Da Costa Gonzalez, Fiz
Robert, Rene
Quere, Claudie
Wikfors, Gary H.
Soudant, Philippe
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| Data: |
2015-05
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| Ano: |
2015
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| Palavras-chave: |
Fatty acid
Larvae
Lipids
Metabolism
Oyster
Synthesis
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| Resumo: |
Essential fatty acids (EFA) are important for bivalve larval survival and growth. The purpose of this study was to quantitatively assess for the first time through a mass-balance approach dietary EFA incorporation and synthesis within Crassostrea gigas larvae. A first experiment was carried out using two microalgae, Tisochrysis lutea (T) and Chaetoceros neogracile (Cg), as mono- and bi-specific diets. A second experiment using a similar design was performed to confirm and extend the results obtained in the first. Flow-through larval rearing was used for accurate control of food supply and measurement of ingestion. Non-methylene-interrupted fatty acids were synthetized from precursors supplied in the diet: 16:1n-7 and 18:1n-9, mediated by Delta 5 desaturase. Moreover, this Delta 5 desaturase presumably allowed larvae to convert 20:3n-6 and 20:4n-3 to 20:4n-6 and 20:5n-3, respectively, when the product EFA were poorly or not supplied in the diet, as when larvae were fed T exclusively. Under our experimental conditions, none of the diets induced 22:6n-3 synthesis; however, 22:6n-3 incorporation into larval tissues occurred selectively under non-limiting dietary supply to maintain optimal levels in the larvae. This combination of flow-through larval rearing and biochemical analysis of FA levels could be applied to additional dietary experiments to precisely define optimal levels of EFA supply.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
https://archimer.ifremer.fr/doc/00268/37962/36086.pdf
DOI:10.1007/s11745-015-4006-z
https://archimer.ifremer.fr/doc/00268/37962/
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| Editor: |
Springer Heidelberg
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| Formato: |
application/pdf
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| Fonte: |
Lipids (0024-4201) (Springer Heidelberg), 2015-05 , Vol. 50 , N. 5 , P. 503-511
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| Direitos: |
AOCS 2015
info:eu-repo/semantics/openAccess
restricted use
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